Diaporthales genera incertae sedis » Diaporthales genera incertae sedis » Phruensis 

Phruensis brunneispora

Phruensis brunneispora Pinruan

Entymology: From brunneispora, in reference to the brown versicolorous ascospores.

Holotype: THAILAND. NARATHIWAT: Sirindhorn Peat Swamp Forest, on dead trunk of Licuala longecalycata, 12 May 2001, U. Pinruan (Wah 113.1) in BIOTEC Bangkok Herbarium (BBH). Culture of type isolate: BCC 11169.

 

Sexual morph: Ascomata 950–980 µm high, 1.6–1.7 µm diam, deeply immersed, subglobose, black, coriaceous, ostiolate, scattered. Neck up to 7.5 µm long, 0.5 µm diam., central, cylindrical, black, orange at apex. Peridium up to 250 µm thick, comprising 2 layers, outer layer (40–45 µm) parenchymatous, intensely brown and merging with the host cells, inner layer, cells elongate and hyaline (25–30 µm). Paraphyses up to 11.2–15 µm wide at the base, hypha-like, tapering distally, not embedded in a gelatinous matrix. Asci 260–275 x 45–50 µm (x = 265 x 48 µm, n = 25), 8-spored, cylindro-clavate to fusiform, unitunicate, apedicellate, apically rounded, with a refractive, J-, cuboid  subapical ring, 5–6.2 µm high, 3.7–4.2 µm diam, with a faint channel leading to the apex. Ascospores 115–120 x 7.5–8.7 µm (x = 116.5 x 7.8 µm, n = 25), 4-seriate to fasciculate, cylindrical, straight or curved, versicolored, brown with hyaline to pale brown end cells, 9–11-septate, smooth-walled, with minute ephemeral mucilaginous material at the ends. Asexual morph: Phialophora-like anamorph: Colonies (BCC 11169) on PDA reaching 2 cm diam in 7 d at room temperature (22–24 ^OC), effuse, brown mycelium party immersed, nonstromatic. Conidiophores up to 5 µm wide at the base, semimacronematous, mononematous, branched, straight or slightly flexuous, pale brown to brown, smooth. Conidiogenous cells monophialidic, determinate, with small collarettes. Conidia 11.5–14 x 1.5 µm (x = 12 x 1.5 µm, n = 25), aggregated in slimy heads, semi-endogenous, straight or curved, oblong, colorless, smooth, 0-septate. Ascomata not formed in culture. (Description based on Pinruan et al. 2004).

 

Distribution: Thailand, Narathiwat Province, Sirindhorn peat swamp forest, on submerged palm in freshwater stream (Pinruan et al. 2004).

 

Notes: Holotype BBH, Pinruan Wah 113.1. SSU sequence data is available.

 

References

Hyde KD, Norphanphoun C, Maharachchikumbura SSN, Bhat DJ et al. 2020 – Refined families of Sordariomycetes. Mycosphere 11(1): 305–1059.

Luo ZL, Hyde KD, Liu JK, Maharachchikumbura SSN et al. 2019 – Freshwater Sordariomycetes. Fungal Diversity 99, 451–60.

Pinruan U, Sakayaroj J, Jones EBG & Hyde KD. 2004 - Aquatic fungi from peat swamp palms: Phruensis brunneispora gen. et sp. nov. and its hyphomycete anamorph. Mycologia, 96:5, 1163-1170.

Fig 1. Light micrographs of Phruensis brunneispora (from holotype). 1, 2. Ascomata embedded in the substratum with long necks, tips bright orange. 3. Horizontal section of ascoma with outer perithecial wall (arrow OL) dark brown to black, inner layer (arrow IL) of hyaline elongate cells. Scale bars: 1 = 10 µm; 2 = 5 mm; 3 = 30 µm. (Photo taken from Pinruan et al. 2004)

 

Fig 2. Light micrographs of Phruensis brunneispora (from holotype) and Phialophora-like anamorph. 4. Cross section of neck with fine paraphyses. 5, 6. Asci cylindrical-clavate, ascospores 4-seriate. 7, 8. Cuboid subapical ring (arrows). 9. Hyphalike paraphyses. 10–12. Ascospores with 12–13-septae, central cells brown, end cells hyaline to pale brown. 13, 14. Conidiophores with small collarettes (arrows). 15, 16. Conidia. Scale bars: 4 = 40 µm; 5–12 = 20 µm; 13–16 = 10 µm. (Photo taken from Pinruan et al. 2004)[u1] 

 

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