Lindgomyces griseosporus
Lindgomyces griseosporus Y. Zhang ter, J. Fourn. & K.D.Hyde
Index Fungorum number: IF563691
Holotype: BJFC200094
Etymology: From the Latin “griseo”, in reference to the color of the mature ascospores.
Sexual morph: Ascomata 240–290(–330) µm high, 320–350 µm diameter, black with roughened surface, scattered to most often densely gregarious, erumpent, at times coalescent and thus appearing multiloculate, subglobose to broadly conical, often laterally flattened, apex with low rounded to truncate papillae, opening through 50–60 µm wide pores; the wood surrounding the base is often more or less pigmented by dark brown hyphae originating from the ascomatal wall. Peridium 40–70 µm thick, leathery, thinner at the base, composed of two layers of textura angularis, textura prismatica near the base: outer layer 30–50 µm thick, strongly melanized, inner layer 10–25 µm thick, of cells 6–8 µm diameter, pale brown. Hamathecium of dense, 2–3 µm wide, septate, branching, cellular pseudoparaphyses associated with gelatinous material. Asci basal, few, bitunicate, fissitunicate, clavate with a broadly rounded apex and a small truncate ocular chamber visible in young asci, subsessile, 140–180 × 24–30 µm, with eight obliquely uniseriate to 2–3-seriate ascospores; whole asci may be released through the ostiole upon drying. Ascospores 46–53 × 11–12.5 µm (mean ¼ 49.4 = 11.7 µm, n ¼ 60), fusiform, slightly curved with narrowly rounded ends, 1-septate, septum supramedian (upper/lower cell length ¼ 0.42–0.48), constricted at the septum, upper cell broader, with 4–6 large guttules, wall gray while in the ascus, smooth to finely verruculose. Senescent ascospores turn grayish brown, verrucose and 1–3-septate. No sheath or appendages observed in India Ink on mature ascospores, but a well-defined mucilaginous sheath can be seen on immature ascospores, and some mucilaginous remains can be occasionally encountered on mature ascospores. Asexual morph: Undetermined. (Description from Zhang et al. 2014)
Specimens examined: FRANCE, Arie`ge, Rimont, Peyrau, on submerged wood of Alnus glutinosa, 9 October 2006, leg. J. Fournier (BJFC200094, HOLOTYPE ex-type culture, CBS 123100). FRANCE, Castelnau-Durban, Douach, ca. 550 m, on submerged wood of Fraxinus excelsior, 19 February 1998, leg. J. Fournier (MFLU12-0286); Orlu, Orgeix river, 1150 m, on unidentified submerged wood, 26 May 2006, leg. J. Fournier (MFLU12-0287); Rimont, La Maille, ca 550 m, on submerged wood of Alnus glutinosa, 8 July 2007, leg. J. Fournier (MFLU12-0288); Rimont, Peyrau, ca. 400 m, on submerged wood of Rhamnus cathartica, 26 July 2007, leg. J. Fournier (MFLU12-0289); Deux Se`vres, Paizay le Tort, La Bouchonnerie, on submerged wood of Alnus glutinosa, 14 April 2008, leg M. Delpont & J. Fournier (MFLU12-0290); Haute Garonne, Avignonet, Port Lauragais, Canal du Midi, on submerged wood of Frangula alnus, 23 November 2006, leg. M. Delpont (MFLU12-0291); same locality, on submerged dead root of Platanus sp., 26 November 2006, leg. M. Delpont (MFLU12-0292); Estadens, Les Mouchous, on unidentified submerged wood, 12 May 2007, leg. J. Fournier (MFLU12-0293); Izaut de l’Hotel, Le Job Stream, on submerged twig of Alnus glutinosa, 12 May 2007, leg. J. Fournier (MFLU12-0294); Morbihan, La Gacilly, Les Bresles, on submerged wood of Salix sp., 20 April 2008, J. P. Priou (MFLU12-0295); Saint Martin d’Oust, Rieux, on unidentified submerged wood, 25 October 2010, leg. det. Y. Mourgues (MFLU12-0296); Saoˆne et Loire: La Grande Verrie`re, Le Me´chet Brook, on unidentified submerged wood, 3 July 2009, leg. JP Dechaume, det. A. Gardiennet (MFLU12-0297); Loire Atlantique, La Chapelle sur Erdre, La Joneliere, Erdre river, 29 July 2012, on submerged wood, leg. Pascal Ribollet (BJFC 200007); Orvault, Bois Ragnenet, small tributary of Cens river, 22 August 2012, on submerged wood, leg. Pascal Ribollet (BJFC 200008); SPAIN, Asturias, Bimeda, 43°5'33.24"N, 6°31'53.70"W, on submerged wood, 22 May 2009, leg. J. Linde (MFLU12-0298); Baselgas, 43°18'33.02"N, 6°03'35.03"W, 27 March 2010, leg. J. Linde (MFLU12-0299).
Morphology: See Zhang et al. (2014)
Freshwater distribution: France and Spain (Zhang et al. 2014)
Notes: Morphologically, L. griseosporus is similar to other members of the genus in having small erumpent ascomata that are often gregarious, with a relatively thick and leathery wall and a faintly papillate and porate apex; microscopically, its subsessile clavate asci nested in cellular pseudoparaphyses and its large 2-celled ascospores match those encountered in the type species L. ingoldianus and other species recently included in this genus. However, L. griseosporus can be easily distinguished from all known species in the genus in having gray ascospores that lack a sheath or appendages at maturity. In addition to their characteristic color which is already present in ascospores still in the ascus, they are distinctive in being relatively narrow with a marked supramedian septum and narrowly rounded sometimes subacute ends. In the cladogram, L. griseosporus is sibling to all other species of Lindgomyces, and its conspecific relationship with any other species of Lindgomyces is not supported in the study of Zhang et al. (2014) .
Based on numerous collections in various regions of France and Spain, L. griseosporus appears to be a common and widespread species and has a unique habitat occurrence. It tends to be common in streams, creeks and rivulets flowing in acidic or neutral soils, and to be much rarer in calcareous regions, but its ecological requirements may depend on several still unknown other factors. For example, its occurrence in Canal du Midi, almost a lentic habitat in water heavily polluted by organic matter was unexpected. Like most of aquatic lignicolous ascomycetes, L. griseosporus has a wide range of hosts but has so far not been recorded on coniferous wood. (Notes from Zhang et al. 2014)
References
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