Pleosporales » Lophiostomataceae » Crassiclypeus

Crassiclypeus aquaticus

Crassiclypeus aquaticus A. Hashim., K. Hiray. & Kaz. Tanaka

Index Fungorum number: IF 823132

Holotype: HHUF 27985

Etymology: Refers to its aquatic habitat.

 

Sexual morph: Ascomata subglobose, 3–5 grouped, immersed, dark brown to black, 400–780 μm high, 600–1000 μm diam. Ostiolar neck crest-like, elongated and laterally compressed, 80–100 μm high, 160–300 μm wide, composed of 2.5–7 μm diam, globose, thick-walled, brown to black cells, with hyaline periphyses, surrounded by a well-developed clypeus (up to 390 μm wide). Peridium uniform, (30–)45–70 μm thick at side, composed of 8–9(–15) layers of elongated, thin-walled, 5–18 × 2.5–5 μm, brown cells, surrounded by brown hyphae. Pseudoparaphyses numerous, 1.5–2 μm wide, septate, branched and anastomosed. Asci bitunicate, fissitunicate, clavate, 85–125 × 11–17.5 μm (x̅ = 102.5 × 12.9 μm, n = 96), with a stipe (14–50 μm long, x̅ = 26.3 μm, n = 24), apically rounded with a broad ocular chamber, 8-spored. Ascospores fusiform with obtuse ends, 20–32.5 × 5–8 μm (x̅ = 25.3 × 6.7 μm, n = 190), l/w 2.9–4.8 (x̅ = 3.8, n = 190), hyaline, with a septum nearly median (0.42–)0.45–0.55, x̅ = 0.49, n = 167), slightly constricted at the septum, smooth, with a narrow sheath. Sheath drawn out 2–5 μm long at both ends, with an internal chamber at both ends of ascospores. Asexual morph: Conidiomata pycnidial, globose to subglobose, up to 165 μm high, 135–180 μm diam, scattered to 3–6 grouped, superficial to immersed. Peridium 11.5–18.5 μm thick, composed of 2–4 layers of 8–13.5 × 3–4 μm, subglobose to rectangular, brown cells. Conidiophores reduced to conidiogenous cells. Conidiogenous cells phialidic, 6–12 × 2.5–4 μm, ampliform, hyaline, smooth. Conidia subglobose with rounded ends, 2–3(–4) × 1.2–1.8 μm (x̅ = 2.4 × 1.5 μm, n = 60), l/w 1.2–2.2 (x̅ = 1.6, n = 60), hyaline, smooth, aseptate, guttulate when young. (Description from Hashimoto et al. 2018)

 

Material examined (all on submerged dead twigs of woody plant): JAPAN, Aomori, Hirosaki, Aoki, near Mohei-pond, 7 Dec. 2002, K. Tanaka & N. Asama, KT 970 (HHUF 27985 holotype designated here; ex-holotype culture CBS 143643 = JCM 13087 = MAFF 239597); ibid., 21 Jul. 2007, K. Hirayama & K. Tanaka, KH 56 (HHUF 30566; ex-paratype culture CBS 143639); ibid., 23 Sep. 2007, K. Hirayama & K. Tanaka, KH 91 (HHUF 30567; ex-paratype culture CBS 143640); ibid., 29 Oct. 2007, K. Hirayama & K. Tanaka, KH 104 (HHUF 30568; ex-paratype culture CBS 143641); ibid., 30 Aug. 2008, K. Hirayama & K. Tanaka, KH 185 (HHUF 30569; ex-paratype culture CBS 143642).

 

Notes: Crassiclypeus aquaticus was collected from submerged dead twigs of woody plants during summer, fall, and winter. Crassiclypeus aquaticus strains produced a red pigment in water agar medium. (Notes from Hashimoto et al. 2018)

 

Freshwater Distribution: Japan (Hashimoto et al. 2018)

 

Fig. 1 Crassiclypeus aquaticus A–C Appearance of ascomata on substrate. D–F Ascomata in longitudinal section. G Peridium of ascoma. H Ostiolar neck of ascoma. I Ascus apex. J Ascus stipe. K, L Asci. M Pseudoparaphyses. N–S Ascospores (arrowheads indicate an internal chamber in S). T–V Conidiomata in culture. W Conidioma in longitudinal section. X Peridium of conidioma. Y–AA Conidiogenous cells. AB, AC Conidia. A, G–L, O–S from HHUF 30569. B–F from HHUF 27985 (holotype); M, N from HHUF 30567; T–AC from culture CBS 143640. Scale bars: A, T = 1 mm; B = 300 μm; C, U, V = 100 μm; D–F = 50 μm; K–M, W = 10 μm; G–J, N–S, X–AC = 5 μm. (Hashimoto et al. 2018)

 

References

Hashimoto A, Hirayama K, Takahashi H, Matsumura M, Okada G, Chen CY, Huang JW, Kakishima M, Ono T, Tanaka K (2018) Resolving the Lophiostoma bipolare complex: generic delimitations within Lophiostomataceae. Stud Mycol 90:161–189. https://doi.org/10.1016/j.simyco.2018.03.001.

 

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