Pleosporales » Trematosphaeriaceae » Falciformispora

Falciformispora uttaraditensis

Falciformispora uttaraditensis Boonmee & Huanraluek

Index Fungorum number: IF 557030; Facesoffungi number: FoF 06508

Etymology: Named after Uttaradit Province, Thailand, where this fungus was collected.

Holotype: MFLU 19-2361

 

Saprobic on the surface of submerged twigs in tidal creeks. Sexual morph: Ascomata 148–268 μm high × 110–224 μm diam. (x̄ = 208 × 179 μm, n = 5), immersed to erumpent, solitary, scattered on substrate, subglobose to globose, coriaceous, dark brown. Peridium 16–44 μm wide, 5 layers of dark brown cells of textura angularis, almost black at outside. Hamathecium with 2 μm wide, anastomosing, septate, branched pseudoparaphyses, hyaline. Asci 54–108 × 13–27 μm (x̄ = 73 × 18 μm, n = 4), 8-spored, bitunicate, cylindrical-clavate to broadly obpyriform, straight or slightly curved, sessile. Ascospores 28–59 × 6–14 μm (x̄ = 41 × 20 μm, n = 20), 2–3-seriate, fusiform to clavate, slightly curved, tapering towards narrow ends, 6-septate, obviously multi-guttulate when immature, hyaline, surrounded by a distinct mucilaginous sheath. Asexual morph: Undetermined. (Descriptions from Hyde et al. 2020)

 

Culture characteristics: Ascospores germinating on MEA within 24 h at room temperature and germ tubes produced from the ends of the ascospore. Colonies on PDA reaching 2 cm diam. for one month at 25 °C. Initially aerial mycelium white, slightly raised, becoming grey to dark grey when old, convex with papillate on surface, crenated, green to dark brown from below, white to light yellow margin. (Descriptions from Hyde et al. 2020)

 

Material examined: THAILAND, Uttaradit Province, Phichai District, Thasak, Ban Cham Tok, on submerged twigs in tidal creeks, 29 December 2018, S. Boonmee, CTU6 (MFLU 19-2361, holotype), ex-type living culture, MFLUCC 19-0485.

 

Notes: Falciformispora uttaraditensis resembles F. aquatica D.F. Bao, K.D. Hyde & H.Y. Su in having immersed, coriaceous ascomata in wood tissue, dark pigmented, ostiolate, cylindro-clavate to broadly obpyriform asci, and fusiform to clavate, multi-septate, hyaline ascospores, surrounded by a distinct mucilaginous sheath (Hyde et al. 2019). However, F. uttaraditensis differs from F. aquatica in having smaller ascomata, asci and ascospores. Furthermore, phylogenetic analyses indicate that F. uttaraditensis forms a basal lineage in the clade with high statistic support (92%ML, 0.99 BYPP). To justify our new species, we follow the guidelines of Jeewon and Hyde (2016). Comparison of 546 nucleotides of the ITS sequences between F. uttaraditensis (MFLUCC 19-0485) and F. aquatica (MFLUCC 18-0212) reveals 20 nucleotide differences (3.8%), which is sufficient to delineate our new species. (Notes from Hyde et al. 2020)

 

Freshwater distribution: Thailand (Hyde et al. 2020)

 

Fig 1. Falciformispora uttaraditensis (MFLU 19-0485, holotype). a. Appearance of ascomata on wood substrate. b. Vertical section through ascoma. c. Close up of peridium. f. Pseudoparaphyses. e–g. Asci. h, i. Ascospores. j. Ascospore with sheath in nigrosine reagent. k. Germinated spore. l, m. Culture on PDA after 4 weeks from surface and reverse. Scale bars: a = 500 µm, b = 100 µm, c = 20 µm, d = 20 µm, e–g = 50 µm, h–j = 20 µm, k = 50 µm (Hyde et al. 2020)

 

References

Hyde KD, Danushka S, Tennakoon DS, Jeewon R, Bhat DJ, Maharachchikumbura SSN, Rossi W, Leonardi M, Lee HB, Mun HY, Houbraken J, Nguyen TTT, Jeon SJ, Frisvad JC, Dhanushka N, Wanasinghe DN, Luücking R, Aptroot A, Cáceres MES, Karunarathna SC, Hongsanan S, Phookamsak R, de Silva NI, Thambugala KM, Jayawardena RS, Senanayake IC, Boonmee S, Chen J, Luo ZL, Phukhamsakda C, Pereira OL, Abreu VP, Rosado AWC, Bart B, Randrianjohany E, Hofstetter V, Gibertoni TB, da Silva Soares AM, Plautz HL Jr, Sotão HMP, Xavier WKS, Bezerra JDP, de Oliveira TGL, de Souza-Motta CM, Magalhães OMC, Bundhun D, Harishchandra D, Manawasinghe IS, Dong W, Zhang SN, Bao DF, Samarakoon MC, Pem D, Karunarathna A, Lin CG, Yang J, Perera RH, Kumar V, Huang SK, Dayarathne MC, Ekanayaka AH, Jayasiri SC, Xiao YP, Konta S, Niskanen T, Liimatainen K, Dai YC, Ji XH, Tian XM, Mešić A, Singh SK, Phutthacharoen K, Cai L, Sorvongxay T, Thiyagaraja V, Norphanphoun C, Chaiwan N, Lu YZ, Jiang HB, Zhang JF, Abeywickrama PD, Aluthmuhandiram JVS, Brahmanage RS, Zeng M, Chethana T, Wei DP, Réblová M, Fournier J, Nekvindová J, do Nascimento Barbosa R, dos Santos JEF, de Oliveira NT, Li GJ, Ertz D, Shang QJ, Phillips AJL, Kuo CH, Camporesi E, Bulgakov TS, Lumyong S, Jones EBG, Chomnunti P, Gentekaki E, Bungartz F, Zeng XY, Fryar S, Tkalčec Z, Liang J, Li GS, Wen TC, Singh PN, Gafforov Y, Promputtha I, Yasanthika E, Goonasekara ID, Zhao RL, Zhao Q, Kirk PM, Liu JK, Yan JY, Mortimer PE, Xu JC (2019) Fungal diversity notes 1036–1150: taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Divers 96:1–242

Hyde KD, Dong Y, Phookamsak R, Jeewon R, Bhat DJ, Jones EBG, Liu NG, Abeywickrama PD, Mapook A, Wei D, Perera RH, Manawasinghe IS, Pem D, Bundhun D, Karunarathna A, Ekanayaka AH, Bao DF, Li J, Samarakoon MC, Chaiwan N, Lin CG, Phutthacharoen K, Zhang SN, Senanayake IC, Goonasekara ID, Thambugala KM, Phukhamsakda C, Tennakoon DS, Jiang HB, Yang J, Zeng M, Huanraluek N, Liu JK, Wijesinghe SN, Tian Q, Tibpromma S, Brahmanage RS, Boonmee S, Huang SK, Thiyagaraja V, Lu YZ, Jayawardena RS, Dong W, Yang EF, Singh SK, Singh SM, Rana S, Lad SS, Anand G, Devadatha B, Niranjan M, Sarma VV, Liimatainen K, Aguirre-Hudson B, Niskanen T, Overall A, Alvarenga RLM, Gibertoni TB, Pfliegler WP, Horváth E, Imre A, Alves AL, da Silva Santos AC, Tiago PV, Bulgakov TS, Wanasinghe DN, Bahkali AH, Doilom M, Elgorban AM, Maharachchikumbura SSN, Rajeshkumar KC, Haelewaters D, Mortimer PE, Zhao Q, Lumyong S, Xu J, Sheng J (2020) Fungal diversity notes 1151–1276: taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Divers 100:5–277

Jeewon R, Hyde KD (2016) Establishing species boundaries and new taxa among fungi: recommendations to resolve taxonomic ambiguities. Mycosphere 7:1669–1677

 

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