Neochlamydotubeufia khunkornensis
Neochlamydotubeufia khunkornensis (Boonmee & K.D. Hyde) Y.Z. Lu
≡ Chlamydotubeufia khunkornensis Boonmee & K.D. Hyde, Fungal Diversity 51(1): 80 (2011)
Index Fungorum number: IF 554863; Facesoffungi number: FoF 04733
Saprobic on submerged wood from freshwater habitats. Ascomata 180–250 μm high × 199–249 μm diam (x̄ =210.5 × 220μm, n = 5), superficial, solitary or scattered, globose to subglobose, with a central ostiolate 20–30 μm wide, covered with dark brown setae, which taper to an acute apex. Peridium 18–28 μm thick, composed of 3–5 layers of light brown cells of compressed textura angularis. Hamathecium numerous, cellular, cylindrical, filiform pseudoparaphyses, hyaline, embedded in a gelatinous matrix. Asci 79.5–106 × 13–21 μm (x̄ =96.5×15.5μm, n = 20), 8-spored, bitunicate, cylindrical to clavate, apically rounded, ocular chamber not observed, short pedicellate. Ascospores 34–42 × 5–7 μm (x̄ =38×6μm, n = 20), 2–3-seriate, fusiform, broad at the supra-median cell, 6–7-septate, ends asymmetrical, not constricted at any septum, hyaline, smooth walled. Asexual morph (in culture): Hyphomycetous, chlamydosporous. Chlamydospores 50–135 μm long, 30–80 μm wide (x̄ = 85 × 55 μm, n = 50), holoblastic, sometimes intercalary, broadly oval to ellipsoid, dictyoseptate, pale brown when immature, darkened to black when matured, with terminal cells round and pale brown.
Culture characteristics: Conidia germinating on water agar and producing germ tubes within 36 h. Colonies growing slowly on malt extract agar (MEA), circular, with flat surface, veined and without wrinkle, edge entire, reaching 21 mm in 6 weeks at 28 °C, brown to dark brown in MEA media. Mycelium superficial and partially immersed, branched, septate, hyaline to pale brown, becoming dark brown due to the development of chlamydospores.
Material examined: THAILAND, Chiang Rai, Muang, Ban Nang Lae Nai, on submerged decaying wood in a freshwater stream, 28 November 2015, Saranyaphat Boonmee and Yong-Zhong Lu, TUB05–1 (MFLU 17–1167 = HKAS 100723), living culture, MFLUCC 16–0025; Ibid., TUB03–4 (MFLU 17–1170), living culture, MFLUCC 16–1129; THAILAND, Krabi, Muang, Khao Khram, Ban Nong Jig, Tha Pom Klong Song Nam, on submerged decaying wood in freshwater stream, 16 December 2015, Saranyaphat Boonmee, TP01–5 (MFLU 17–1181), living culture, MFLUCC 16–1126.
Notes: Morphologically, our new collections found from decaying wood substrate have typical chlamydospores, which are similar to Chlamydotubeufia huaikangplaensis and Dictyospora thailandica (Brahamanage et al. 2017; Hyde et al. 2017). Phylogenetically, these three newly obtained isolates form a monotypic clade with two known Chlamydotubeufia khunkornensis isolates and can be recognized as one species. Furthermore, our phylogenetic result shows that this taxon shares a sister relationship to Neochlamydotubeufia fusiformis but distantly related to Chlamydotubeufia and Dictyospora members, therefore, we synonymize it as Neochlamydotubeufia khunkornensis. (Lu et al. 2018)
Freshwater distribution: Thailand (Lu et al. 2018)
Fig 1 Chlamydotubeufia khunkornensis (MFLU10–0051, holotype). a Superficial ascomata on substrate. Note ascomata covered with dark brown setae. b, c Section through ascoma and peridium that comprises brown-walled cells of textura angularis. d Pseudoparaphyses, cellular, embedded in a mucilaginous matrix. e-g Asci at young and mature stages. h–l Ascospores. Scale bars: a, b = 100 μm, c, e–g = 20 μm, d = 5 μm, h–l = 10 μm (Boonmee et al. 2011)
Fig 2 Chlamydotubeufia khunkornensis (MFLUCC10–0118, holotype). Colonies on MEA. a Germinating ascospore. b, c Colonies on MEA from surface and in reverse. Note darkened colonies. d–f Mycelium development on culture. g–k Dictyochlamydospores. Scale bars: a–c = 10 mm, d–f = 10 μm, g–k = 20 μm (Boonmee et al. 2011)
Fig 3 The asexual morph of Neochlamydotubeufia khunkornensis from natural decaying wood substrata. a Chlamydospores on natural substrata. b, c Conidiogenous cells with attached conidia. d–o Chlamydospores. Scale bars: a = 200 µm, b–o = 20 µm. Notes a–g MFLU 17–1167, h–k MFLU 17–1170, l–o MFLU 17–1181 (Lu et al. 2018)
Fig 4 The asexual morph of Neochlamydotubeufia khunkornensis from cultures. a Germinating chlamydospore. b, c Colony on MEA from above and below. d Conidiogenous cells with attached conidia. e–o Conidiogenous cells with attached immature chlamydospores. p Matured chlamydospore. Scale bars: a, d = 50 µm, e–p = 20 µm (Lu et al. 2018)
References
Boonmee S, Zhang Y, Chomnunti P, Chukeatirote E, Tsui CKM, Bahkali AH, Hyde KD (2011) Revision of lignicolous Tubeufiaceae based on morphological reexamination and phylogenetic analysis. Fungal Divers 51:63–102. https://doi.org/10.1007/s13225-011-0147-4
Brahmanage RS, Lu YZ, Bhat DJ, Wanasinghe DN, Yan JY, Hyde KD, Boonmee S (2017) Phylogenetic investigations on freshwater fungi in Tubeufiaceae (Tubeufiales) reveals the new genus Dictyospora and new species Chlamydotubeufia aquatica and Helicosporium flavum. Mycosphere 8:917–933. https://doi.org/10.5943/MYCOSPHERE/8/7/8
Hyde KD, Norphanphoun C, Abreu VP, Bazzicalupo A, Thilini Chethana KW, Clericuzio M, Dayarathne MC, Dissanayake AJ, Ekanayaka AH, He MQ, Hongsanan S, Huang SK, Jayasiri SC, Jayawardena RS, Karunarathna A, Konta S, Kušan I, Lee H, Li J, Lin CG, Liu NG, Lu YZ, Luo ZL, Manawasinghe IS, Mapook A, Perera RH, Phookamsak R, Phukhamsakda C, Siedlecki I, Soares AM, Tennakoon DS, Tian Q, Tibpromma S, Wanasinghe DN, Xiao YP, Yang J, Zeng XY, Abdel-Aziz FA, Li WJ, Senanayake IC, Shang QJ, Daranagama DA, de Silva NI, Thambugala KM, Abdel-Wahab MA, Bahkali AH, Berbee ML, Boonmee S, Bhat DJ, Bulgakov TS, Buyck B, Camporesi E, Castañeda-Ruiz RF, Chomnunti P, Doilom M, Dovana F, Gibertoni TB, Jadan M, Jeewon R, Jones EBG, Kang JC, Karunarathna SC, Lim YW, Liu JK, Liu ZY, Plautz HL, Lumyong S, Maharachchikumbura SSN, Matočec N, McKenzie EHC, Mešić A, Miller D, Pawłowska J, Pereira OL, Promputtha I, Romero AI, Ryvarden L, Su HY, Suetrong S, Tkalčec Z, Vizzini A, Wen TC, Wisitrassameewong K, Wrzosek M, Xu JC, Zhao Q, Zhao RL, Mortimer PE (2017) Fungal diversity notes 603–708: taxonomic and phylogenetic notes on genera and species. Fungal Divers 87:1–235. https://doi.org/10.1007/s13225-017-0391-3
Lu YZ, Liu JK (Jack), Hyde KD, Jeewon R, Kang JC, Fan C, Boonmee S, Bhat DJ, Luo ZL, Lin CG, Eungwanichayapant PD (2018) A taxonomic reassessment of Tubeufiales based on multi-locus phylogeny and morphology. Fungal Divers 92:131–344. https://doi.org/10.1007/s13225-018-0411-y
Recent Genus
1Neochlamydotubeufia
Neohelicomyces
Recent Species
Neohelicomyces submersusNeohelicomyces hyalosporus
Neohelicomyces grandisporus